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The search for more yeast diversity drove the team from the University of Manchester to search for new yeasts at higher altitudes, as the vast majority of known yeasts derive from lower areas. Dozens of different yeast samples were collected from oak trees, a particularly rich source of yeasts, and surrounding soils, and cold-tolerant strains were isolated based on their ability to grow at lower temperatures than most other yeasts. The Manchester scientists then teamed up with specialists in yeast taxonomy at the NCYC to sift through what they had found.
For each person or group: 6 test tubes; test tube rack; 100 mL beaker; styrofoam cup (not as tall as the test tubes that you are using); larger beaker or other container to steady the styrofoam cup; thermometer; balance that can weigh gram amounts; 25 mL graduated cylinder; a teaspoon of rapid rise yeast; sugar; water; at least ten balloons that fit tightly over the test tube mouth (smaller balloons sizes are better; long and thin is better than round); vinegar; ammonia; liquid plant fertilizer; liquid bleach; small cups or beakers; heat source; pipettes or eyedroppers.